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KMID : 1094720200250020255
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Biotechnology and Bioprocess Engineering
2020 Volume.25 No. 2 p.255 ~ p.263
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Simple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beverages
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Hidayat Mochammad Amrun
Maharani Diah Ayu
Purwanto Djoko Agus
Kuswandi Bambang
Yuwono Mochammad
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Abstract
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A simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method.
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KEYWORD
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biosensor, tyrosinase, MBTH, TPC, green tea, scanometric
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